The present work seeks to quantify the knowledge of two rural communities in the semi-arid region of the state of Pernambuco (northeastern Brazil) concerning two species of native medicinal plants: “aroeira do sertao”, ˜ Myracrodruon urundeuva (Engl.) Fr. All. (Anacardiaceae) and “angico”, Anadenanthera colubrina (Vell.) Brenan (Mimosaceae). Semi-structured interviews were carried out, combined with a checklist/inventory method, which yielded different indexes for quantifying knowledge and use of these species. In addition, the reliability of local knowledge was tested using analytical techniques to determine actual tannin concentrations. Although both communities possess knowledge concerning these two species, one of them stood out in terms of the diversity of information presented (P < 0.05), a difference that may be related to its lower degree of modernization. In general, older people had a greater variety of information about these plants, although both men and women demonstrated similar knowledge. Of the 101 people interviewed in Riachao, 85% stated that they knew of uses for both species studied; in Ameixas, of the 55 interviewees, 63% ˜ responded that they knew of uses for Myracrodruon urundeuva, and 45% knew of uses for Anadenanthera colubrina. A total of 97 different uses were reported by all informants for the two species studied. Of these, 62 were mentioned in only a single community, confirming our hypothesis of differences in knowledge between them. Informants from both communities knew of a great variety of uses for these plants as well as a number of different collection techniques. We expected that knowledge about the two species would differ in relation to both gender and age, but this was only true for one of the communities. Greater concentrations of tannins were expected to be found consistently in the tree bark, but experimental data demonstrated that tannin concentrations can vary among plant parts during the year.