BioMed Central Page 1 of 12 (page number not for citation purposes) Journal of Ethnobiology and Ethnomedicine Research Open Access Ethnopharmacological survey of Samburu district, Kenya Mark O Nanyingi*1, James M Mbaria1, Adamson L Lanyasunya2, Cyrus G Wagate1, Kipsengeret B Koros1,3, Humphrey F Kaburia1, Rahab W Munenge1 and William O Ogara1 Address: 1Department of Public health Pharmacology and Toxicology, University of Nairobi, P.O BOX 29053-00625 Nairobi, Kenya, 2Samburu Integrated Resource Aid Network (SIRAN), P.O BOX 26 Maralal, Kenya and 3Center for Public Health Research, Kenya Medical Research Institute, P.O. Box 54840, 00200 Nairobi, Kenya Email: Mark O Nanyingi* - mnanyingi@gmail.com; James M Mbaria - james.mbaria@uonbi.ac.ke; Adamson L Lanyasunya - adamsonlanya@yahoo.com; Cyrus G Wagate - gwagate@yahoo.com; Kipsengeret B Koros - Bkoros@kemrinuitm.or.ke; Humphrey F Kaburia - hkaburia@uonbi.ac.ke; Rahab W Munenge - rmunenge@uonbi.ac.ke; William O Ogara - wogara@uonbi.ac.ke * Corresponding author Abstract Background: Ethnobotanical pharmacopoeia is confidently used in disease intervention and there is need for documentation and preservation of traditional medical knowledge to bolster the discovery of novel drugs. The objective of the present study was to document the indigenous medicinal plant utilization, management and their extinction threats in Samburu District, Kenya. Methods: Field research was conducted in six divisions of Samburu District in Kenya. We randomly sampled 100 consented interviewees stratified by age, gender, occupation and level of education. We collected plant use data through semi-structured questionnaires; transect walks, oral interviews and focus groups discussions. Voucher specimens of all cited botanic species were collected and deposited at University of Nairobi's botany herbarium. Results: Data on plant use from the informants yielded 990 citations on 56 medicinal plant species, which are used to treat 54 different animal and human diseases including; malaria, digestive disorders, respiratory syndromes and ectoparasites. Conclusion: The ethnomedicinal use of plant species was documented in the study area for treatment of both human and veterinary diseases. The local population has high ethnobotanical knowledge and has adopted sound management conservation practices. The major threatening factors reported were anthropogenic and natural. Ethnomedical documentation and sustainable plant utilization can support drug discovery efforts in developing countries. Background The Samburu pastoralists of Kenya are still among the traditional communities of the country that have retained most of their knowledge about the use of a large part of the plants in their environment for a wide variety of purposes. This knowledge is however dwindling rapidly due to changes towards a more western lifestyle, overgrazing and overexploitation of plant resources have already led to a decline of the plant material available [1]. Published: 23 May 2008 Journal of Ethnobiology and Ethnomedicine 2008, 4:14 doi:10.1186/1746-4269-4-14 Received: 4 March 2008 Accepted: 23 May 2008 This article is available from: http://www.ethnobiomed.com/content/4/1/14 © 2008 Nanyingi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 2 of 12 (page number not for citation purposes) Ethnopharmacology and natural product drug discovery remains a significant hope in the improving the poor livelihoods of rural communities. Many modern pharmaceuticals have their origin in ethnomedicine and ethnoveterinary medicine, which relies upon a local pharmacopoeia [2]. The ethnopharmacology knowledge is a holistic system approach that can serve as an innovative and powerful discovery engines for newer, safer and affordable medicines [3]. High throughput screening in industries and the isolation of many have proven to be of poor cost-effectiveness due to lack of comprehensive biological and clinical evaluation [4]. Natural products from botanical sources used in traditional medicine may combat multidrug-resistant (MDR) infectious diseases through the elucidation and validation of biological compounds with novel mechanisms of action[5]. Ethnobotanical and ethnopharmacological studies normally involve field explorations of indigenous medical knowledge and biodiversity [6]. The cultural importance of traditional medicine and physical isolation of communities both in general and from primary health cares (PHCs), are the factors that influence the dramatic use of use herbal medicines in developing countries[7,8]. Cultural acceptability of traditional practices, along with perceptions of affordability, safety and efficacy play a role in stimulating scientific research and validation of traditional medicines [9]. Ethnoveterinary medicine (EVM) include use of medicinal plants, surgical techniques and management practices [10] which forms a basis of veterinary diseases management in Samburu District. Herbal medicines are cheap and readily available in the pastoral areas but lack of sufficient scientific data on efficacy, therapeutic index, toxic effects and other pharmacological and toxicological properties to support their use [11]. Despite the fact that EVM has been very crucial for the animal healthcares of most developing countries, it has not yet been well documented and much effort is needed in research and integration activities in these countries [12]. Interdisciplinary studies to effectively combine ethnography, medical anthropology and ethnopharmacology to formulate meaningful conclusions regarding how local healers effect cure should be encouraged [13]. There are several ethnomedicinal and ethnoveterinary studies which are being carried out realizing the benefit of traditional medication to promote the cheap and safe disease management. The outcomes of these researches have immense contribution to attitude change and adaptation, though there are very few in light of Kenya's biodiversity. The population in the District depend on livestock products for their food source and the natural vegetation as source of fuel, medicine, construction materials and other cultural needs. The overdependence on natural vegetation as food, fuel, building and medicine in Samburu District might be the cause of the cause for the environmental. Therefore, there is a need to carry out more research pertaining to documentation of useful medicinal plants in this area before they disappear, especially those which are already endangered by natural and anthropogenic activities [14-16]. In the current study we investigated and documented the local use of medicinal plants, management and extinction threats. We also compared the use of medicinal plants in treatment of human and animal diseases. Materials and methods Study area and ethnographic background Samburu District is situated in the northern half of the Rift Valley Province in Kenya. It is bordered by five other Districts; Turkana (Northwest), Baringo (Southwest), Marsabit (Northeast), Isiolo (East) and Laikipia (South) respectively. It lies between Latitudes 0°40" north and 2°50" north of the equator and Longitudes 36°'20" east and 38° 10" east of the Prime Meridian (figure 1). It covers approximately 21126.5 square kilometres. It is divided into six divisions, 39 locations and 108 sub locations. It is characterised by high level plateaus, hills and the Rift valley with an altitude up to 2000 m a.s.l. The study area has a bimodal rainfall distribution from April to May (long rains) and July to September (short rains). The dry season then extends from January to March. The mean annual rainfall is 500 mm. it has mean annual temperatures of 29°C. Samburus are the indigenous dominant ethnic group with Turkana and Maasai having settled in the area. Pastoralism is the major economic activity of the local people. The District has a population of approximately 156125 people[17]. These rural communities are almost totally dependent on forests and savannah as traditional/herbal medicine for their own health and livestock care. Methods A reconnaissance survey was made from December 2006 to January 2007 to obtain an impression on vegetation Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 3 of 12 (page number not for citation purposes) characteristics of the study area. The fieldwork was done in January, February, May and August 2007. A total of 100 informants in figure 2 were selected purposively [18] based on knowledge, attitudes and practices (KAP) survey with the help of local administrators. They included 14 traditional medicine practitioners (4 females and 10 males), 86 locals (Male: Female = 2:1). Information on knowledge depth of respondents was collected from local elderly people, opinion leaders and the local administrators. Similar responses obtained from the three groups were used to identify knowledgeable traditional healers. The respondents and traditional healers identiMap of research area Figure 1 Map of research area. Right: Map of Kenya illustrating the geographical position of Samburu District. Left: Samburu District indicating the divisional administrative boundaries. Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 4 of 12 (page number not for citation purposes) fied were consented to share their knowledge only for the purpose of this study [19]. The methods used for ethnobotanical data collection were semi structured interviews, field observation, preference ranking and direct-matrix ranking according to [18,20]. These interviews were conducted in vernacular (Samburu) translated by local field assistants, three different field trips were conducted. Table 2 indicates relevant data collected on: age, sex, and occupation of informants as well as animal and human health indications treated, vernacular plant names, growth form, plant part used, methods of preparation, dosages, route of administration and possible contraindications. Threats to medicinal plants, conservation efforts, beliefs and indigenous knowledge transfer were also documented. These interviews were done in the field in order to avoid the probable confusions with regard to the identity of the medicinal plants [12]. The morphological characteristics, habitats and habits of medicinal plants were observed, photographed and recorded during and after the interviews. The key informants for purposes of ranking these species were selected randomly from among all informants [21]. Geographical Positioning Systems readings were also taken at the sites where each medicinal plant was collected (GARMIN, Olathe, USA). Some of the plants were identified in the field by herbalists while most were identified at the Herbarium of University of Nairobi, Botany department using specific taxonomic keys and floras[22,23]. Data analyses Ethnobotanical data were entered in to Excel spreadsheet and summarized using descriptive statistics [24]. Number of male and female info trict Figure 2 rmants grouped according to age category in the sample from Kirisia division in Samburu DisNumber of male and female informants grouped according to age category in the sample from Kirisia division in Samburu District. Aggregated data from January-February 2007(dry season) and May-August 2007(rainy season) (N = 100 Informants). Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 5 of 12 (page number not for citation purposes) Wilcoxon's test was used to determine if there was a difference age of respondents and knowledge of medicinal plants used. Chi-square test was used to evaluate the average number of medicinal plant species reported and used by each informant, to determine if there is any significant difference between female and male practitioners with respect to the knowledge and use of medicinal plants. The Spearman rank correlation test was used to determine whether there was a significant correlation between the disease reported and the number of ethnoveterinary medicinal plant species used by each informant for management of the disease. STATA 9.2 IE (Stata Corporation, College Station, Texas, USA) software was used. Results and discussion Medicinal plants diversity and Ethnobotanical knowledge There was a highly significant difference between age of respondents and knowledge of medicinal plants (Wilcoxon's test, p < 0.001). The average number of medicinal plants known and used by female and male practitioners was similar (χ2 = 8.262, d.f. = 13, p = 0.932). It was observed that informants between 58 and 77 years old mentioned more species than younger informants: 58–67 years old: about 10 per informant; 48–57: about 6; 38–47: about 5; and 28–37: 2 quoted plants, due to larger experience of older individuals. These results also agree with other previous studies[25]. It was observed that some plants had more than one vernacular name due to use of the Maasai and Turkana dialects in the area. Diseases treated in the study area A total of 28 animal and 26 human ailments were reported by the informants respectively. The frequency of the most cited ailments and the number of medicinal plant species used are also given in figure 3. The most frequently cited animal health problems were; Retained afterbirth (9), Ectoparasites (8), gastrointestinal disorders (5), Theileriosis (4) and helminthosis (3). Human ailments treated cited frequently included; Malaria (18), gastrointestinal disorders (10), helminthosis (5) and rheumatism (4). Respondents had good knowledge and remote diagnosis of the disease and could readily distinguish them on the basis of accepted signs and symptoms. Ailments such as convulsions, hypertension, asthma, yellow fever and infertility were beyond the scope of the present study, it was considered important to record plants that were frequently mentioned for the treatment of such health conditions (see table 1). Ticks (lntunturi) were the main cattle ectoparasites that the local people controlled using traditional plant extracts. The most frequently used plants for tick control were found to be: Acokanthera schimperi (Lmorijoi), Adenium obesum (Lperantai), Aloe secundiflora (Sukuroi),Psiadia punctulata (Labaai),Nicotiana tabacam (Lkumbao), Euphorbia herechroma (Lpara) represented in table 1. The respondents classified all intestinal worms under one local name, ntubui and therefore use the same plant extracts for all helminths. The main species used for this were: seeds of Myrsine africana L (Seketet) (45%), Albizia anthelmintica (Lmungutan) (30%) and Warburgia ugandensis (Sokorioi) (18%). Medicinal plants used by the locals Fifty four (54) plant species of ethnopharmacological importance were gathered and documented throughout the study period (table 1). These medicinal plants were distributed among 50 genera and 33 families. Analysis of the growth forms of these medicinal plants revealed that, shrubs constituted the largest number or proportion with 31 species (56%), followed by trees 15 (28 %), herbs 5 (9%) and lianas 4 (7%) respectively shown in figure 4. Ethnobotanical knowledge was passed on by word of mouth. Knowledge of ailments such epilepsy, hypertension, venereal diseases, impotence, was generally restricted to the elders and traditional medicine practitioners represented in figure 5. Leaves were the most frequently used plant parts constituting 4 %, followed by roots (3 0%), stems (10%), fruit/ Frequency citation (n = 990) of therapeutic indications of plant remedies based on inform traditional healers (n = 14) Figure 3 ants knowledge (n = 100) and Frequency citation (n = 990) of therapeutic indications of plant remedies based on informants knowledge (n = 100) and traditional healers (n = 14). Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 6 of 12 (page number not for citation purposes) Table 1: Plants of veterinary and medical utility in Samburu District. Family Species Local name Voucher no. Habit Part used Preparation Therapeutic indications Route Anacardiaceae Acokanthera schimperi Benth. & Hook. Lmorijoi MN 18 TR Leaves Hot decoction Ectoparasites(ticks, fleas, mite) pc Adenium obesum (Forssk.) Lperantai MN 40 SH Stem bark Powdering, Cold decoction Ectoparasites pc Carissa edulis (Forssk.) Vahl Lamuriai MN 6 SH Roots, Leaves, Chewing, hot/cold decoction Theileriosis, helminthosis, rheumatism, Malaria TB, Venereal diseases(VD), Salmonellosis, Heart water po Nerium oleander L MN 33 SH Leaves, Seeds Hot decoction URTI and GIT complications po Rhus natalensis Bernh. ex Kraus Lmisigiyoi MN 8 H Roots, leaves Hot decoction Malaria, fevers, TB po Asclepiadaceae Pentarrhinum inspidum E. Mey. Lkisuchie MN 5 L Leaves Hot decoction Anaplasmosis po Asphodelaceae Aloe secundiflora Engl. Sukuroi MN 28 H Stem Burning and squeezing to drip hot exudate Ectoparasites pc Asteraceae Gutenbergia cordifolia Benth. Lodwaporo MN 52 SH Leaves, Roots powdering, hot decoction Ticks, Giardisis Po, pc Psiadia punctulata (DC.) Oliv. & Hiern Labaai MN 19 H Leaves Fumigation, smoke/ steam bath Ectoparasites pc Balanitaceae Balanites rotundifolia (Tiegh.) Sorai, ebei MN 36 SH Leaves Hot decoction GIT complications (Emetic), Eye infection po Boraginaceae Cordia sinensis Lam Lkweite MN 49 SH Flowers Grinding, hot decoction Malaria and fevers, Eye infection po Ehretia buxifolia. Willd Lkinyl MN 2 SH Root bark Pounding, hot decoction GIT complications, URTI, Malaria po Caesalpiniaceae Senna singueana Del Senetoi MN 21 SH Leaves Grinding, hot decoction Malaria, complicated fevers po Canellaceae Warburgia ugandensis Sprague Sokorioi MN 37 TR Stem bark, leaves Hot decoction helminthosis, Heart water, Ectoparasites black quarter, emetic, Trypanosomosis, ECF po Capparidaceae Capparis spinosa.L Lkaridangai MN 15 SH Root bark Hot decoction URTI po Celastraceae Maytenus senegalensis (Lam.) Laimurunyai MN 47 SH Leaves, roots Hot decoction Malaria po Combretaceae Terminalia brownii Fries Lbukoi MN 32 TR Stem bark Hot decoction yellow fever, GIT Complications(emetic), Trypanosomosis po Ebenaceae Euclea divinorum Hiern Lchingei MN 11 SH Seeds, Roots Hot decoction Malaria, Fevers, Anaplasmosis, VD po Euphorbiaceae Croton megalocarpus Hutch. Lmargwet MN 26 TR Root bark Homogenization and decoction Malaria, Fevers, diarrhea, Anaplasmosis, wounds. po Euphorbia candelabrum Mpopong'i MN 44 TR Leaves, barks Stem cutting to drip Sap mix with rumen contents URTI and GIT complications, wounds, coenurosis. pc Euphorbia herechroma Pax lpara MN 53 SH Stem sap Stem cutting to drip Sap Tick infestation, Ectoparasites pc Ricinus communis L. oldula MN 46 SH Leaves Hot decoction Malaria, fevers, RAB Lamiaceae Ajuga remota.Benth Lmenang'i MN 3 H leaves, roots Crushing, hot decoction with soup RAB, GIT complications, Anaplasmosis, Mastitis po Loranthaceae Odontella fischeri Larrudenyai MN 43 TR Stem bark Hot decoction Retained afterbirth, Wounds po Meliaceae Azadirachta indica A. Juss. Mwarubaini MN 29 TR Leaves, Stem barks Stem cutting, pounding, Hot/cold decoction Malaria, Fevers, GIT complications po Mimosaceae Acacia abyssinica Hochst. Lngingiletome MN 45 TR Root bark Homogenization Gastrointestinal distress, Lumbago and arthritis po Acacia drepanolobium Rangau MN 51 TR Root bark Powdering, Cold decoction RAB, Babesiosis, GIT complications Acacia nilotica (L.) Willd. Eluai MN 16 TR Root bark Hot decoction GIT complications, Babesiosis po Albizia anthelmintica Brongn. Lmungutan MN 27 TR Root bark, roots Pounding, Cold decoction Antihelmintic (Lungworms), Malaria, wounds po Myrsinaceae Myrsine africana L. Seketet MN 7 SH Seeds Grinding, hot decoction, chewing Helminthosis, Malaria, Wounds, TB, GIT complications po Rapanea melanophloeios.L Sitoni MN 48 SH Seeds Grinding, hot decoction Antihelmintic (Roundworms). po Myrtaceae Syzygium cordatum Hochst Loiragi MN 39 SH Leaves Hot decoction GIT complications po Olacaceae Ximenia caffra Sond. Ledat MN 13 SH Roots, leaves Hot decoction Malaria, Fevers, Acute URTI, Dermatitis, ulcers, po Oleaceae Olea africana Miller Lgeriyoi MN 23 SH Stem bark Pounding, hot decoction Helmithosis, Asthma, Rheumatism, Lumbago, po Schrebera alata Lkauwawa MN 35 SH Root bark Pounding, chewing Candidiasis, Toothache po Poacea Enteropogon macrostachyus (Hochst.) Lkujita-ongo MN 14 L whole plant Hot decoction Tryapanosomosis po Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 7 of 12 (page number not for citation purposes) seeds (8%) and whole plant (4%) in figure 6. The majority of informants (42%) mentioned Myrsine africana L. (Seketet) as medicinal for the treatment of various animal and human ailments.Seketet was thus the most popular remedy in the study area, followed by Carissa edulis F. (Lamuriai) (5%), Salvadora persica L. (Sekotei) (30%), Albizia anthelmintica Brongn. (Lmungutan) (27%) and Clerodendrum myricoides Hochst. (Lmakutikuti) (22%). The number of species frequently used in each family was cited as; Apocynaceae (6), Mimosaceae (5), Euphorbiaceae and Rhamnaceae (4) and Asteraceae (2) other families were represented by at most one species shown in figure 7. The preparation of the medicines employed several methods; hot decoction (48%) followed by cold decoction (19.4%) and homogenization by pounding or powdering (6.5%) respectively in (table 1). The majority of these preparations were drawn from mixtures of different plant species for the treatment of a single ailment. Oral administration (8%) was the predominant route of administration followed by dermal and nasal administrations (20%). Medicinal plants extinction threats Many medicinal plants in the study area were mainly threatened by anthropogenic and natural factors. The majority of medicinal plants declined due to deforestation for construction, tools, firewood, fodder, agricultural expansion and ceremonial purposes. Drought, overgrazing, bush fires had reportedly affected a significant number of medicinal plant species. Conservation efforts and indigenous knowledge transfer About 47% of the informants had sufficient awareness in conserving some medicinal plant species that were relatively scarce in their surroundings. In situ protection of plants (fencing plants in their natural habitat, refraining from excessive cutting, debarking and uprooting and protection from fire) and ex situ conservation by cultivation of some plants as live fence and in nurseries were undertaken by the locals. Moreover, some of them were keen to inform responsible bodies or authorities of any illegal logging, deforestation and bush fires. Majority of local healers preferred to collect medicinal plants solely to preserve their secrecy sometimes accompanied by the chosen family member(s). The ethnobotanical knowledge is transferred to that trustworthy family Podocarpaceae Podocarpus falcatus (Thunb.) Masanduku MN 38 SH Leaves Hot decoction, Fumigation Measles pc Rhamnaceae Cissus quadrangularis.L Sukurtuti MN 50 SH Leaves, Fruits Crushing, homogenizing for hot/cold decoction Wounds, gastric ulcers, schistosomiasis, neurosis, ECF, rheumatism, epilepsy, TB, Asthma, collibacillosis po Helinus integrifolius (Lam.) Kuntze Lmekori MN 12 SH Root bark Grinding. hot decoction, mix with milk Arthritis, paralysis po Rhamnus stado L Lkukulai MN 10 SH leaves, fruits hot decoction Malaria, fevers po Scutia myrtina (Burm. F.) Kuntz Laturdiai MN 20 SH Leaves Hot decoction Retained afterbirth po Rubiaceae Rubia cordifolia L. Loitunenei MN 9 L Leaves, Roots Hot decoction URTI po Rutaceae Teclea simplicifolia (Engl.) Lgelai MN 42 TR Ro, Flowers Hot decoction Cerebral malaria, Fevers po Zanthoxylum usambarense (Engl.) Loisuk MN 31 SH Seeds Grinding, Hot decoction URTI, Malaria, Malignant catarrhal fever. po Salvadoraceae Salvadora persica L Sekotei MN 41 SH Roots Grinding, hot decoction RAB, ulcers, seizures, toothbrush, mange, Trypanosomosis, Brucellosis, and Anthrax. po Simaroubaceae Harrisonia abyssinica Oliv. Lasaramai MN 24 SH Roots, Leaves Grinding, hot decoction Abscess, ECF, Malaria, Lumbago, Rheumatism, RAB po Solanaceae Nicotiana tabacum L. Lkumbao MN 34 SH Leaves Crushing, smoke bath, chewing Snuff, Ectoparasites, wounds, Babesiosis, gastro-enteritis, chronic cough, gingivitis, candidosis, glossitis Pc, Solanum incanum L. Ltulelei MN 22 H Fruit Burn and drip sap on skin Ectoparasites pc Verbanaceae Lippia javanica (Bur) Sunoni MN 4 SH Leaves Fumigation, decoction Migraines, Measles in, pc Clerodendrum myricoides (Hochst.) Lmakutikuti MN 25 TR Root Powdering, hot decoction, chewing GIT, Lumbago, Venereal diseases. po Viscaeae Viscum tuberculatam Larrudenyai MN54 SH Root bark Hot decoction RAB po Vitaceae Rhoicissus tridentata (L.F.) Nkilenyai MN 1 L Leaves Crushing, cold homogenization URTI, Malaria Hilderbrantia sepalosa Nyirman MN 30 SH Roots Crushing, hot decoction URTI and GIT complications Po Habit (H – Herb, L – Liana, SH – Shrub, TR – Tree); Therapeutic indications (ECF – East Cost Fever, GIT – Gastrointestinal, RAB – Retained Afterbirth, TB – Tuberculosis, URTI – Upper Respiratory Tract Infections, VD – Venereal Diseases); Routes (in -Intranasal, pc – Per cutaneous, po – Per os). Table 1: Plants of veterinary and medical utility in Samburu District. (Continued) Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 8 of 12 (page number not for citation purposes) member by word of mouth rather than through a well organized written script [23]. Some of the ethnopractitioners were reluctant to pass on their plant use knowledge even to their families leading to the fragmentation and loss of the indigenous knowledge system and eventually medicinal plants [12]. This study revealed that traditional medical healers and pastoralists in Samburu District had sound knowledge of traditional medicine, from whom about 54 indications (animal and human) and a total of 56 medicinal plants of importance were recorded. The continued reliance of Samburus' on traditional medicines is due to economic circumstances, which place modern health facilities, services and pharmaceuticals out of the reach of the majority of the population. It is also attributable to the widespread belief in the effectiveness of many traditional therapies [27]. The current investigation indicates that leaves are the most collected plant parts for medicinal purposes and this situation could be a severe threat to some rare and slowly reproducing medicinal plants. The practice of exploiting perennial plant parts, such as roots of relatively slow growing woody species, can result in a decline in both, the size and distributions of populations of the exploited species, and ultimately result in the local extinction of these populations [28]. The use of plants is evenly distributed for management of both medical and veterinary conditions this finding was contrary to earlier findings in the same geographical zone which reported insignificant veterinary use [1]. The majority of plant preparations were drawn from mixtures of different plant species for the treatment of a single ailment and similar results had reported elsewhere [26]. This was contrary to the findings of other researchers in other countries where most of the remedies were prepared Table 2: Data acquisition questionnaire for utilization and conservation of medicinal plants in Samburu district, Kenya QUESTIONNAIRE PART 1: RESPONDENTS DETAILS Name...............................................................Sex....M/F Age...........Years. Occupation........................................................ Level of education........................................ Location/Residence............................................................................................................................... Efficacy/Toxicity Data Type of Plant (Local name).......................................................................................... Preparation method(s).......................................................................................... Administration form (s).......................................................................................... Part(s) of plant used.......................................................................................... Used on : Humans................................... Animals/Species......................................... Route(s) of application.......................................................................................... Approximate dosage.......................................................................................... Response of Patient Good.........................Fair........................ Poor......................... Duration of response Seconds......................... Minutes......................... Hours......................... Complications PART 2: RESPONDENTS CONSENT AGREEMENT I.......................................................................................Hereby agree to participate in this study with my full consent and conscious and declare that to the best of my Knowledge the information that I have provided is true, accurate and complete. Signature/Thumb print...........................................Date............./May/2007 PART 3: RESEARCHER'S DECLARATION 1. The following research will be undertaken with respect to the indigenous knowledge and intellectual proprietary of the Samburu Community. 2. We will at no given time initiate or conduct practices that are deemed to obtain information from the respondents by intimidation, coercion or false pretence. 3. The respondents will be informed of the intended project elaborately prior to questionnaire administration and in confidential to eliminate any degree of conspiracy. 4. We will be no under any obligation to edit or tamper the information provided by the respondents. 5. Translation and transcription will be necessary for clarification due to the language barrier. 6. The information collected will be used for the described research purpose and not any undisclosed any undisclosed intentions. Signatory Researchers: 1. Dr. Nanyingi M.O..................2. Dr. Ogara O.W..................3. Dr. Mbaria J.M.................. Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 9 of 12 (page number not for citation purposes) from a single species [29]. This could also be ascribed to the differences in the socio cultural landscapes, indigenous knowledge on synergetic effect of different medicinal plants and vegetation types in the current study area [29]. The most frequently used methods of preparation were hot decoctions, cold decoctions, powdering and grinding respectively. The prepared medicines were mainly administered through oral (98%), dermal (1.5%), and nasal (0.5%), routes concurring with the previous findings in Ethiopia [30]. The measurements used to determine the dosages were not standardized and depended on the age and physical appearance of the patient, sociocultural explanation of the illness, diagnosis and experience of individual herbalist [27]. The naming of diseases by local people when compared to conventional systems, at times did not distinguish between diseases and symptoms of diseases. This is because local disease nomenclature is based on symptoms of diseases and not according to aetiological information [31,32]. While conducting this study, some informants raised some concern on false promises about getting the feedback. They agreed that scientific methods are better in Percentage distribution of Pl trict Figure 6 ant parts used in Samburu DisPercentage distribution of Plant parts used in Samburu District. Percentage distribution of the nal plants Figure 4 habit growth forms of mediciPercentage distribution of the habit growth forms of medicinal plants. L.( Figure 5 Lewaso Aplea (66 years), The most revered and knowledgea- ble of the remaining traditional healers in Samburu District displaying, Seketet) during the field collection Ximenia caffra Sond.(Ledat) and Myrsine africana Lewaso Aplea (66 years), The most revered and knowledgeable of the remaining traditional healers in Samburu District displaying, Ximenia caffra Sond.(Ledat) and Myrsine africana L.(Seketet) during the field collection. Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 10 of 12 (page number not for citation purposes) revealing harmful effects of herbs. In the drug development research and bioprospection, biological activity based on ethnomedical uses seems as a better approach compared to randomly selected plants [33] We are currently undertaking field trials, in-vitro and invivo tests of these plants for antihelmintic, antiparasitic, antiplasmodial, antibacterial and cytotoxic activities to confirm the therapeutic properties claimed by informants. Conclusions and recommendations Indigenous knowledge, botanical diversity and ethnopharmacopoeia practices were recorded from Samburu District. The botanical resources were found to be under threat due to several anthropogenic and natural factors. Disappearance of traditional medical skills was evident in the study area and this prompts for design of linguistic, anthropological and ethnographic methods in the context of ethnopharmacology to document the indigenous Species frequency of major plant fa Figure 7 milies used in Samburu District Species frequency of major plant families used in Samburu District. Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 11 of 12 (page number not for citation purposes) knowledge so as to minimize the eminent fragmentation and biodiversity loss. The lack of standardized posology of the traditional medicines should encourage pharmacological and toxicological tests to develop formulations that can be administered in measurable dosages whose clinical efficacy can be monitored and pharmacovigilance mechanisms instituted to eliminate development of resistance to these novel compounds. Scientific feedback studies should be encouraged to instil confidence in the increasingly suspicious local populations to eliminate the apparent hostility observed among some the informants during the field research. The data presented in this paper form a basis for further ethnopharmacological research in this region especially in studies dealing with efficacy, dosage, quality and toxicology. Those plants found empirically to be particularly effective can be used in preparation of commercial indigenous-based pharmaceuticals. We recommend that ethnopharmacologists project pharmacologic data against a backdrop of medical ethnography and anthropology. Relevant evidence generated from literature review and these biological tests will be passed back in order to improve the proper use of medicinal plants and create a good relationship for future ethnobotanical studies. The local community of Samburu District, Kenya is the owner of the traditional knowledge presented in this paper, consequently any benefits that may arise from the use this knowledge must be shared with them. Competing interests The authors declare that they have no competing interests. Authors' contributions MON carried out the field research, analyzed the data and wrote the manuscript, JMM and WOO designed the study, conducted fieldwork, supervised the research and revised the manuscript, CGW reviewed the manuscript and conducted the field research, KBK reviewed the manuscript and assisted in data analysis, HFK, RWM and AAL assisted in the fieldwork and taxonomic identification of the botanic specimens. All authors read and approved the final manuscript. Acknowledgements The present research was funded by the Commision of Higher Education (CHE), Kenya through a research grant: CHE/1/26/1/4 (PI: J.M.Mbaria). We especially thank all of the study participants who voluntarily shared a wealth of their accumulated knowledge regarding the ethnomedical and ethnoveterinary practices of their communities and for their hospitality. References 1. Bussmann RW: Ethnobotany of the Samburu of Mt. Nyiru, South Turkana, Kenya. Journal of Ethnobiology and Ethnomedicine 2006, 2(35):. 2. Tamboura HH, Sawadogo LL, Kaboré H, Yameogo SM: Ethnoveterinary Medicine and Indigenous Pharmacopoeia of Passoré Province in Burkina Faso. Ann N Y Acad Sci 2000, 916:259-264. 3. Patwardhan B: Ethnopharmacology and drug discovery. Journal of Ethnopharmacology 2005, 100:50-52. 4. Bertrand G, Elaine E, Jacques F: Beyond the myth of expensive clinical study: Assessment of traditional medicines. Journal of Ethnopharmacology 2007, 113:382-386. 5. Cassandra LQ, Andrea P, Bradley CB: Dermatological remedies in the traditional pharmacopoeia of Vulture-Alto Bradano, inland southern Italy. J Ethnobiol Ethnomed 2008, 4(5):. 6. Soejarto DD, Fong HHS, Tan GT, Zhang HJ, Ma CY, Franzblau SG, Gyllenhaal C, Riley MC, Kadushin MR, Pezzuto JM, Xuan LT, Hiep NT, Hung NV, Vu BM, Loc PK, Dac LX, Binh LT, Chien NQ, Hai NV, Bich TQ, Cuong NM, Southavong B, Sydara K, Bouamanivong S, Ly HM, Tran Van Thuy Rose WC, Dietzman GR: Ethnobotany/ethnopharmacology and mass bioprospecting: Issues on intellectual property and benefit-sharing. Journal of Ethnopharmacology 2005, 100:15-22. 7. Vandebroek ICJ, De Jonckheere S, Sanca S, Semo L, Van Damme P, Van Puyvelde L, De Kimpe N: Use of medicinal plants and pharmaceuticals by indigenous communities in the Bolivian Andes and Amazon. Bulletin of the World Health Organization 2004, 82:243-250. 8. Vicente T, Omar M, Paola VF, Giovanni V, Chabaco A, Tomás Z: An ethnobotanical survey of medicinal plants used in Loja and Zamora-Chinchipe, Ecuador. Journal of Ethnopharmacology 2007, 111:63-81. 9. WHO: WHO Traditional Medicine Strategy 2002–2005. In promoting the role of traditional medicine in health care systems: A strategy for the African Region, WHO 2002. 10. Wanyama JB: 1. In Confidently used ethnoveterinary knowledge among pastoralists of Samburu, Kenya: Methodology and Results. Rukangira Volume 1. 1st edition. Nairobi: ITDG Kenya; 1997:82. 11. Gathuma JM, Mbaria JM, Wanyama J, Kaburia HFAMJN, Mpoke L: Efficacy of Myrsine africana, Albizia anthelmintica and Hilderbrantia sepalosa herbal remedies against mixed natural sheep helminthosis in Samburu District, Kenya. Journal of Ethnopharmacology 2004, 91:7-12. 12. Yinenger H, Kelbessa E, Bekele T, Lukelal E: Ethnoveterinary medicinal plants at Bale Mountains National Park, Ethiopia. Journal of Ethnopharmacology 2007, 112:55-70. 13. Etkin NL: Perspectives in ethnopharmacology: forging a closer link between bioscience and traditional empirical knowledge. Journal of Ethnopharmacology 2001, 76:177-182. 14. Ssegawa P, Kasenene JM: Medicinal plant diversity and uses in the Sango bay area, Southern Uganda. Journal of Ethnopharmacology 2007, 113:521-540. 15. Vandebroek I, Thomas E, Sanca S, Van Damme P, Luc Van P, De Kimpe N: Comparison of health conditions treated with traditional and biomedical health care in a Quechua community in rural Bolivia. Journal of Ethnobiology and Ethnomedicine 2008, 4(1):. 16. Maregesi SM, Olipa DN, Luc P, Arnold JV: Ethnopharmacological survey of the Bunda District, Tanzania: Plants used to treat infectious diseases. Journal of Ethnopharmacology 2007, 113:457-470. 17. Central bureau of Statistics: 1999 Population and Housing Census, counting our people for development Ministry of Finance and Planning, Ed.(Government of Kenya); 2001. 18. Martin GJ: Ethnobotany: a methods manual London, UK: Chapman and Hall; 1995. 19. Lulekal E, Kelbessa E, Bekele T, Yineger H: An ethnobotanical study of medicinal plants in Mana Angetu District, southeastern Ethiopia. Journal of Ethnobiology and Ethnomedicine 2008, 4:10. 20. Cotton CM: Ethnobotany: Principles and applications Chichester, New York: John Wiley and Sons Ltd; 1996. 21. Alexiades M: Collecting ethnobotanical data. An introduction to basic and concepts and techniques, in Selected Guideline for Ethnobotanical Research: A Field Manual Edited by: Alexiades M, Sheldon JW. The New York Botanical Garden, USA: New York; 1996:53-94. 22. Bamps P: Catalogue of the phanerogamic families dealt with in the main floras of Tropical Africa. Boissiera 1976, 24:667-686. 23. Polhill RM, (Ed): Flora of Tropical East Africa Edited by: Balkema AA. Rotterdam/Boston; 2006. (Series available in parts under various dates and authors) Publish with BioMed Central and every scientist can read your work free of charge "BioMed Central will be the most significant development for disseminating the results of biomedical research in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — you keep the copyright Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp BioMedcentral Journal of Ethnobiology and Ethnomedicine 2008, 4:14 http://www.ethnobiomed.com/content/4/1/14 Page 12 of 12 (page number not for citation purposes) 24. Höft M, Barik SK, Lykke AM: Quantitative Ethnobotany. Applications of multivariate and statistical analyses in ethnobotany. People and Plant Working Paper 1999. 25. Magassouba FB, Diallo A, Kouyát M, Mara F, Mara O, Bangoura O, Camara A, Traoré S, Diallo AK, Zaoro M, Lamah K, Diallo S, Camara G, Traoré S, Kéita A, Camara MK, Barry R, Kéita S, Oula K, Barry MS, Donzo M, Camara K, Toté K, Vanden Berghe D, Totté J, Pieters L, Vlietinck AJ, Baldé AM: Ethnobotanical survey and antibacterial activity of some plants used in Guinean traditional medicine. Journal of Ethnopharmacology 2007, 114:44-53. 26. Tabuti JRS, Dhillion SS, Lye KA: Traditional medicine in Bulamogi County, Uganda: its practitioners, users and viability. Journal of Ethnopharmacology 2003, 85(2):119-129. 27. Teklehaymanot T, Giday M: Ethnobotanical study of medicinal plants used by people in Zegie Peninsula, Northwestern Ethiopia. J Ethnobiol Ethnomed 2007, 3(12):. 28. Cunningham AB: African medicinal plants: setting priorities at the interface between conservation and primary health care. People and Plants UNESCO : Paris; 1993. 29. Mirutse G, Gobena A: An ethnobotanical survey of plants of veterinary importance in two woredas of southern Tigray, Northern Ethiopia [abstract]. Ethiopian Journal of Science 2003, 26(2):123-136. 30. Kebu B, Ensermu K, Zemede A: Indigenous Medicinal Plant Utilization, Management and Threats in Fentalle Area, Eastern Shewa, Ethiopia [abstract]. Ethiopian Journal of Biological Science 2002, 2(2):143-156. 31. McCorkle M, Mathias E, Sahillhorn TM: Ethnoveterinary Research and Development London: Intermediate Technology Development Group Publications; 1996:338. 32. Jäger AK: Is traditional medicine better 25 years later. Journal of Ethnopharmacology 2005, 100:3-4. 33. Cordell GA: Changing strategies in natural product chemistry. Phytochemistry 1995, 40(6):1585-1612.